Sept62013

Papers: =Multisite Light-Induced Phosphorylation of the Transcription Factor PIF3 Is Necessary for Both Its Rapid Degradation and Concomitant Negative Feedback Modulation of Photoreceptor phyB Levels in// Arabidopsis //[|[C]][|[W]] =  [|+] Author Affiliations  Questions: 1- What are the three affects light has on PIF3? (Hint: all three are examined in the paper) 2- The authors use a truncated form of PIF3 for this study. Why did they use this truncated form and how does this affect their interpretation of results? 3- In Figs. 3C and 3D, is the interpretation of this result completely valid (that the mutations do not affect DNA binding)? Why or why not? 4- Fig 4 - If PIF3 phosphorylation status is not the determinant for nuclear localization, what might be the determinant?
 * 1) Weimin Ni [|a] , [|b] , [|1] ,
 * 2) Shou-Ling Xu [|c] , <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|d] <span class="xref-sep" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: 0.33em;">, <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|1] ,
 * 3) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Robert J. Chalkley <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|c] ,
 * 4) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Thao Nguyen D. Pham <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|a] <span class="xref-sep" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: 0.33em;">, <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|b] ,
 * 5) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Shenheng Guan <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|c] ,
 * 6) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Dave A. Maltby <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|c] ,
 * 7) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Alma L. Burlingame <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|c] ,
 * 8) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Zhi-Yong Wang <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|d] and
 * 9) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: left; vertical-align: baseline; vertical-align: baseline;"><span class="name" style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Peter H. Quail <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|a] <span class="xref-sep" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: 0.33em;">, <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|b] <span class="xref-sep" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: 0.33em;">, <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0.2em;">[|2]
 * 1) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline; vertical-align: baseline;">  a Department of Plant and Microbial Biology, University of California, Berkeley, California 94720
 * 2) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline; vertical-align: baseline;">  b U.S. Department of Agriculture/Agriculture Research Service, Plant Gene Expression Center, Albany, California 94710
 * 3) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline; vertical-align: baseline;">  c Department of Pharmaceutical Chemistry, University of California, San Francisco, California 94143
 * 4) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline; vertical-align: baseline;">  d Department of Plant Biology, Carnegie Institution for Science, Stanford, California 94305
 * 5) <span style="font-family: inherit; font-family: inherit; font-size: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline; vertical-align: baseline;"><span style="color: #006699; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0em;">[|↵] <span class="corresp-label" style="font-family: inherit; font-size: 0.85em; text-align: inherit; vertical-align: super;">2 Address correspondence to <span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: 0em;">quail@berkeley.edu.
 * 1) <span style="display: block; font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;"><span class="slug-metadata-note ahead-of-print" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">Published online before print <span class="slug-ahead-of-print-date" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">July 2013 <span class="slug-metadata-note ahead-of-print" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">, doi: <span class="slug-doi" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;"><span style="color: #006699; font-family: inherit; font-size: inherit; text-align: inherit; text-decoration: none; vertical-align: baseline;">[|http:/​/​dx.​doi.​org/​10.​1105/​tpc.​113.​112342]   The Plant Cell <span class="slug-pub-date" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;"> July 2013 <span class="slug-vol" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">vol. 25 <span class="slug-issue" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">no. 7 <span class="slug-pages" style="font-family: inherit; font-size: inherit; text-align: inherit; vertical-align: baseline;">2679-2698