Sept202013

Papers: Paper #1: Jinek, M., Chylinski, K., Fonfara, I., Hauer, M., Doudna, J. A., and Charpentier, E. “A Programmable Dual-RNA-Guided DNA Endonuclease in Adaptive Bacterial Immunity.” Science (New York, NY) 337, no. 6096 (2012): 816–821. doi:10.1126/science.1225829, Paper #2: Shan, Q., Wang, Y., Li, J., Zhang, Y., Chen, K., Liang, Z., Zhang, K., Liu, J., Xi, J. J., Qiu, J.-L., and Gao, C. “Targeted Genome Modification of Crop Plants Using a CRISPR-Cas System.” Nat Biotechnol 31, no. 8 (2013): 686–688. doi:10.1038/nbt.2650,

Here is a general review: Pennisi, E. “The CRISPR Craze” Science (New York, NY) (2013)

Questions: General: 1. What are the possible applications of genome editing? 2. Would manipulating plants and other organisms by this technology be considered GMO? Paper #1: 3. Can you think of another application for the D10A/H840A Cas9 nuclease? Paper #2: 4. What are the advantages of homologous recombination in genome editing applications?